Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.
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Extraction Shade dried and coarsely powdered leaves of A. Escherichia coli was highly resistant to all the plant extracts at all concentrations.
Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper
The crude extract of A. Cytotoxic A and antimicrobial effects of Methylobacterium sp. Further elution of the column with chloroform: A human adenocarcinoma cancer cell line was obtained from National Institute of Cell Sciences, Pune. Minimum Inhibitory Concentration MIC Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].
Investigation on antifeedant and anthelmentic potential of Adhatoda vasica nees. Essential oils of the leaves of A. Table 2 Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi. The estimation of Vasicine acetate content in the extract was performed using linear regression analysis. National Center for Biotechnology InformationU. BoxRiyadhSaudi Arabia.
Cytotoxicity of each sample was expressed as IC adatoda value.
Ignacimuthu1 adtivity, 23 C. Negative control was prepared using respective solvents. Materials and Methods 2. The spot turned orange red on spraying with Dragendorff’s reagent. The plant is used in the indigenous system of medicine in India and is a well-known expectorant in both Ayurvedic and Unani Systems of Medicine [ 45 ].
The structure of the isolated compound Vasicine and Vasicine acetate Figure 1.
Published online Jan Separation was carried out at room temperature. The column was eluted with chloroform and chloroform: Table 4 Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line. Journal of Obstetrics and Gynaecology of India.
In vitro antibacterial activity of selected medicinal plants from lower Himalayas.
Every year, at leastpeople die worldwide from cancer related to their workplace [ 3 ]. Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper.
Conclusion Vasicine acetate was obtained by acetylation of Vasicine lf from A. Sample ethanol extract Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m. The compound showed prominent cytotoxic activity in vitro against A lung adenocarcinoma cancer cell line. Classical Uses of Medicinal Plants. The plant is reported to show abortifacient [ 7 ], antimicrobial [ 89 ], and antitussive activities [ 10 activityy.
Vasicine acetate showed moderate antibacterial activity compared to Vasicine.
All melting point values are uncorrected and taken by open capillary method on a heating block Instrument. Vasicine acetate showed minimum inhibitory concentration values against bacteria: The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ].
The following Gram negative, Gram positive bacteria, clinical isolates, and fungi were used for the experiment: Abstract Adhatoda vasica L.
Antibacterial activity of adhatoda vasica leaf extract
The extract was filtered through Whatman No. The physical and spectroscopic data were compared with those reported in the literature [ 2122 ]. Minimum vaxica concentration of Vasicine acetate against tested bacteria and fungi.
The plant contains alkaloids such as Vasicine, vasicinone, deoxyvasicine, vasicol, adhatodinine, and vasicinol [ 12 ]. Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica. The combined chloroform extract was washed with water, dipped, and dried over anhydrous sodium sulphate.
Antimicrobial and antimycobacterial activities actiity methyl caffeate isolated from Solanum torvum Swartz. The physical and spectroscopic data were compared with those reported in the literature [ 15 ]. Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. A voucher specimen No. HPLC analysis was carried out on a Waters Alliance separations module with photodiode array detector Waters, Vasicine acetate exhibited good zone of inhibition against bacteria: Vasicine acetate could be checked further in drug discovery programme.
The ethanol extract of A.